Mingji freezing cryo tube factory

The ethanol volatility time must be extended Desiccation is not suitable. Services include: scientific research And clinical grade adenovirus, lentivirus, adeno-associated virus (AAV) packaging, plasmid vector construction, TALEN gene knockout, gene mutation, etc. If there is a precipitate, it can be treated in a 37 ° C water bath. Reach OD600 1.0; (2) Solution Ⅱ 0. Alkali lysis is the most widely used method for preparing plasmid DNA. After adding solution RNase, it should be stored at 4 ° C to prevent enzyme inactivation.2Ml NaOH / 1% SDS; (3) plastic ware suppliers Solution Ⅲ 3Ml potassium acetate / 2Ml acetic acid (4) isopropanol, absolute ethanol,

75% ethanol should be stored in a refrigerator at -20 ° C for pre-cooling; phenol chloroform is stored in a refrigerator at 4 ° C for pre-storage; Incubate overnight at 37 ° C. Note: Weizhen Biological is committed to providing high-quality virus packaging services for scientific researchers. The first two methods are more violent and are applicable to smaller plasmids (<15Kb). Warm, generally used for plasmids with large molecular weight (> 15Kb). (4)

Discard the supernatant and use 1ml of pre-chilled 75% ethanol. After scribing, pick the plasmid extraction solution contaminated with the monoclonal shake reagent and reconfigure it, and then use the solution after autoclaving.2ml of solution I (RNase has been added), and mix thoroughly, add 0.0-12. Sincerely welcome your consultation and purchase!. Bacterial aging. After inversion, centrifuge at 13000rcf for 1min.Experimental principle There are currently three commonly used plasmid extraction methods: alkaline lysis, boiling and detergent lysis.

The elution volume is small. Experimental procedure 1. Centrifuge at 10000rcf for 10min. Note If the strain is a Gram-positive bacterium, the bacterium has a layer of cell wall, and lysozyme must be added to dissolve it. Under the premise of ensuring the plasmid concentration, increase the volume of the eluate.5ml EP tube, add an equal volume of phenol-chloroform mixed solution, and mix thoroughly.4 ml solution III, mix by inversion, and centrifuge at 13000rcf for 30min. 2. So far, the company has a human-sourced spot plasmid library (18 000), an adenovirus spot library (12 000), and an adeno-associated virus (AAV) spot library. (5) Discard the supernatant, add 1ml of pre-chilled 75% ethanol along the wall, and discard directly